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I do have a question about the security of my web app. I am running experiment using my pythonanywhere web app and require access to participants' microphones.

However, the participants' browsers do not allow my web app to access the microphone. It seems that neither google chrome nor safari allows access. There is no allow option for users to choose on browser.

How to make my web app seem secure for the browser?

It on the client's machine, between the browser and the os. It has nothing to do with what you do on our servers.