Thanks for the response, I have deployed the python application on python anywhere, after clicking on the Reload of the Web tab of python anywhere. HTML pages are not rendered, I checked the access.log, error.log, Server log, but i could not see any error on access.log, error.log. I tried running the default flask_app.py, but got the same issue.
But in the Server log I below message are of concern as per my analysis.
2021-06-26 05:00:09 *** uWSGI is running in multiple interpreter mode ***
2021-06-26 05:00:09 gracefully (RE)spawned uWSGI master process (pid: 1)
2021-06-26 05:00:09 spawned uWSGI worker 1 (pid: 4, cores: 1)
2021-06-26 05:00:09 metrics collector thread started
2021-06-26 05:00:09 spawned 2 offload threads for uWSGI worker 1
2021-06-26 05:00:11 announcing my loyalty to the Emperor...
2021-06-26 05:07:41 Sat Jun 26 05:07:41 2021 - *** /var/www/rishi2512_pythonanywhere_com_wsgi.py has been touched... grace them all !!! ***
2021-06-26 05:07:41 ...gracefully killing workers...
2021-06-26 05:07:41 Gracefully killing worker 1 (pid: 4)..